| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 10-May-2009 |  David Worrall | Shrewsbury Town | England | 12-Jun-1990 | 18(333) |
| 07-May-2009 |  Josh Wright | Gillingham | England | 06-Nov-1989 | 19(182) |
| 10-May-2009 |  Joe Thompson | Rochdale | England | 05-Mar-1989 | 20(66) |
| 07-May-2009 |  Elliott Bennett | Bury | England | 18-Dec-1988 | 20(140) |
| 07-May-2009 | William Buckley | Rochdale | England | 12-Aug-1988 | 20(268) |
| 07-May-2009 | Frank Fielding | Rochdale | England | 04-Apr-1988 | 21(33) |
| 07-May-2009 | Luke Daniels | Shrewsbury Town | England | 05-Jan-1988 | 21(123) |
| 10-May-2009 | Danny Racchi | Bury | England | 22-Nov-1987 | 21(170) |
| 07-May-2009 | Stuart Lewis | Gillingham | England | 15-Oct-1987 | 21(205) |
| 07-May-2009 | Chris Humphrey | Shrewsbury Town | Jamaica | 19-Sep-1987 | 21(231) |
| 07-May-2009 | Mike Jones | Bury | England | 15-Aug-1987 | 21(266) |
| 10-May-2009 | Moses Ashikodi | Shrewsbury Town | Antigua and Barbuda | 27-Jun-1987 | 21(318) |
| 07-May-2009 | Rory McArdle | Rochdale | Northern Ireland | 01-May-1987 | 22(7) |
| 07-May-2009 | Simeon Jackson | Gillingham | Canada | 28-Mar-1987 | 22(41) |
| 07-May-2009 | Curtis Weston | Gillingham | England | 24-Jan-1987 | 22(104) |
| 07-May-2009 | Andy Barcham | Gillingham | England | 16-Dec-1986 | 22(143) |
| 07-May-2009 | Adam Le Fondre | Rochdale | England | 02-Dec-1986 | 22(157) |
| 07-May-2009 | Garry Richards | Gillingham | England | 11-Jun-1986 | 22(331) |
| 07-May-2009 | David Buchanan | Bury | Northern Ireland | 06-May-1986 | 23(1) |
| 07-May-2009 | Chris Dagnall | Rochdale | England | 15-Apr-1986 | 23(22) |
| 07-May-2009 | Stephen Dawson | Bury | Republic of Ireland | 04-Dec-1985 | 23(154) |
| 07-May-2009 | Tom Kennedy | Rochdale | England | 24-Jun-1985 | 23(317) |
| 07-May-2009 | Neil Ashton | Shrewsbury Town | England | 15-Apr-1985 | 24(22) |
| 07-May-2009 | Kelvin Langmead | Shrewsbury Town | England | 23-Mar-1985 | 24(45) |
| 07-May-2009 | Albert Jarrett | Gillingham | Sierra Leone | 23-Oct-1984 | 24(196) |
| 07-May-2009 | Barry Fuller | Gillingham | England | 25-Sep-1984 | 24(224) |
| 07-May-2009 | Adam Rundle | Rochdale | England | 08-Jul-1984 | 24(303) |
| 07-May-2009 | Dennis Oli | Gillingham | England | 28-Jan-1984 | 25(100) |
| 07-May-2009 | John Welsh | Bury | England | 10-Jan-1984 | 25(118) |
| 10-May-2009 | Jon Shaw | Rochdale | England | 14-Nov-1983 | 25(178) |
| 07-May-2009 | Simon King | Gillingham | England | 11-Apr-1983 | 26(27) |
| 07-May-2009 | Nick Chadwick | Shrewsbury Town | England | 26-Oct-1982 | 26(194) |
| 07-May-2009 | Andy Bishop | Bury | England | 19-Oct-1982 | 26(201) |
| 07-May-2009 | John Nutter | Gillingham | England | 13-Jun-1982 | 26(329) |
| 07-May-2009 | Simon Ramsden | Rochdale | England | 17-Dec-1981 | 27(141) |
| 07-May-2009 | Ciaran Toner | Rochdale | Northern Ireland | 30-Jun-1981 | 27(311) |
| 07-May-2009 | Ben Futcher | Bury | England | 04-Jun-1981 | 27(337) |
| 07-May-2009 | Ben Davies | Shrewsbury Town | England | 27-May-1981 | 27(345) |
| 07-May-2009 | Darren Moss | Shrewsbury Town | Wales | 24-May-1981 | 27(348) |
| 07-May-2009 | Nathan Stanton | Rochdale | England | 06-May-1981 | 28(1) |
| 07-May-2009 | Grant Holt | Shrewsbury Town | England | 12-Apr-1981 | 28(25) |
| 07-May-2009 | Omer Riza | Shrewsbury Town | Turkey | 08-Nov-1979 | 29(181) |
| 07-May-2009 | Steve Haslam | Bury | England | 06-Sep-1979 | 29(244) |
| 07-May-2009 | Phillip Jevons | Bury | England | 01-Aug-1979 | 29(280) |
| 07-May-2009 | Mark McCammon | Gillingham | Barbados | 07-Aug-1978 | 30(274) |
| 07-May-2009 | Brian Barry-Murphy | Bury | Republic of Ireland | 27-Jul-1978 | 30(285) |
| 10-May-2009 | Mark Bentley | Gillingham | England | 07-Jan-1978 | 31(123) |
| 07-May-2009 | Kevin McIntyre | Shrewsbury Town | England | 23-Dec-1977 | 31(136) |
| 07-May-2009 | Gary Jones | Rochdale | England | 03-Jun-1977 | 31(339) |
| 07-May-2009 | Wayne Brown | Bury | England | 14-Jan-1977 | 32(113) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).