| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 15-May-2016 |  Conor Chaplin | Portsmouth | England | 16-Feb-1997 | 19(88) |
| 15-May-2016 | Ben Close | Portsmouth | England | 08-Aug-1996 | 19(280) |
| 12-May-2016 |  Ben Purrington | Plymouth Argyle | England | 05-May-1996 | 20(7) |
| 12-May-2016 | Hiram Boateng | Plymouth Argyle | England | 08-Jan-1996 | 20(125) |
| 18-May-2016 |  Bradley Halliday | Accrington Stanley | England | 10-Jul-1995 | 20(313) |
| 14-May-2016 | Tom Beere | AFC Wimbledon | England | 27-Jan-1995 | 21(108) |
| 12-May-2016 | Craig Tanner | Plymouth Argyle | England | 27-Oct-1994 | 21(198) |
| 14-May-2016 | Ross Etheridge | Accrington Stanley | England | 14-Sep-1994 | 21(243) |
| 14-May-2016 | Shay McCartan | Accrington Stanley | Northern Ireland | 18-May-1994 | 21(362) |
| 14-May-2016 | Josh Windass | Accrington Stanley | England | 09-Jan-1994 | 22(126) |
| 14-May-2016 | Adebayo Azeez | AFC Wimbledon | England | 08-Jan-1994 | 22(127) |
| 30-May-2016 | Jordon Forster | Plymouth Argyle | Scotland | 23-Sep-1993 | 22(250) |
| 14-May-2016 | Matty Pearson | Accrington Stanley | England | 03-Aug-1993 | 22(285) |
| 14-May-2016 | Jake Reeves | AFC Wimbledon | England | 30-May-1993 | 22(350) |
| 12-May-2016 | Curtis Nelson | Plymouth Argyle | England | 21-May-1993 | 22(357) |
| 12-May-2016 | Carl McHugh | Plymouth Argyle | Republic of Ireland | 05-Feb-1993 | 23(96) |
| 30-May-2016 | Connor Smith | AFC Wimbledon | Republic of Ireland | 18-Feb-1993 | 23(101) |
| 12-May-2016 | Marc McNulty | Portsmouth | Scotland | 14-Sep-1992 | 23(240) |
| 12-May-2016 | Ryan Allsop | Portsmouth | England | 17-Jun-1992 | 23(329) |
| 14-May-2016 | Kelle Roos | AFC Wimbledon | Netherlands | 31-May-1992 | 23(348) |
| 14-May-2016 | Adam Buxton | Accrington Stanley | England | 12-May-1992 | 24(2) |
| 14-May-2016 | Tom Davies | Accrington Stanley | England | 18-Apr-1992 | 24(26) |
| 30-May-2016 | Jonathan Meades | AFC Wimbledon | Wales | 02-Mar-1992 | 24(89) |
| 14-May-2016 | Piero Mingoia | Accrington Stanley | England | 20-Oct-1991 | 24(207) |
| 12-May-2016 | Christian Burgess | Portsmouth | England | 07-Oct-1991 | 24(218) |
| 12-May-2016 | Jake Jervis | Plymouth Argyle | England | 17-Sep-1991 | 24(238) |
| 12-May-2016 | Gregg Wylde | Plymouth Argyle | Scotland | 23-Mar-1991 | 25(51) |
| 12-May-2016 | Kelvin Mellor | Plymouth Argyle | England | 25-Jan-1991 | 25(108) |
| 12-May-2016 | Adam Barton | Portsmouth | Northern Ireland | 07-Jan-1991 | 25(126) |
| 12-May-2016 | Jamille Matt | Plymouth Argyle | England | 02-Dec-1990 | 25(162) |
| 14-May-2016 | Billy Kee | Accrington Stanley | Northern Ireland | 01-Dec-1990 | 25(165) |
| 14-May-2016 | Tom Elliott | AFC Wimbledon | England | 09-Nov-1990 | 25(187) |
| 12-May-2016 | Kyle Bennett | Portsmouth | England | 09-Sep-1990 | 25(246) |
| 12-May-2016 | Enda Stevens | Portsmouth | Republic of Ireland | 09-Jul-1990 | 25(308) |
| 14-May-2016 | Lyle Taylor | AFC Wimbledon | England | 29-Mar-1990 | 26(47) |
| 14-May-2016 | Terry Gornell | Accrington Stanley | England | 16-Dec-1989 | 26(150) |
| 14-May-2016 | Callum Kennedy | AFC Wimbledon | England | 09-Nov-1989 | 26(187) |
| 12-May-2016 | Graham Carey | Plymouth Argyle | Republic of Ireland | 20-May-1989 | 26(358) |
| 14-May-2016 | Sean McConville | Accrington Stanley | England | 06-Mar-1989 | 27(69) |
| 12-May-2016 | Adam McGurk | Portsmouth | Northern Ireland | 24-Jan-1989 | 27(108) |
| 14-May-2016 | Sean Rigg | AFC Wimbledon | England | 01-Oct-1988 | 27(225) |
| 30-May-2016 | Reuben Reid | Plymouth Argyle | England | 26-Jul-1988 | 27(308) |
| 14-May-2016 | Seamus Conneely | Accrington Stanley | Republic of Ireland | 09-Jul-1988 | 27(309) |
| 12-May-2016 | Gareth Evans | Portsmouth | England | 26-Apr-1988 | 28(16) |
| 12-May-2016 | Peter Hartley | Plymouth Argyle | England | 03-Apr-1988 | 28(39) |
| 14-May-2016 | Darius Charles | AFC Wimbledon | England | 10-Dec-1987 | 28(156) |
| 14-May-2016 | Andy Barcham | AFC Wimbledon | England | 16-Dec-1986 | 29(150) |
| 14-May-2016 | Mark Hughes | Accrington Stanley | England | 09-Dec-1986 | 29(157) |
| 12-May-2016 | Danny Hollands | Portsmouth | England | 06-Nov-1985 | 30(188) |
| 12-May-2016 | Gary Sawyer | Plymouth Argyle | England | 05-Jul-1985 | 30(312) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).