| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 08-May-2009 |  Kyle Walker | Sheffield United | England | 28-May-1990 | 18(346) |
| 09-May-2009 |  Jay Rodriguez | Burnley | England | 29-Jul-1989 | 19(284) |
| 12-May-2009 |  Simon Church | Reading | Wales | 10-Dec-1988 | 20(153) |
| 08-May-2009 | Kyle Naughton | Sheffield United | England | 11-Nov-1988 | 20(178) |
| 12-May-2009 | Alex Pearce | Reading | Republic of Ireland | 09-Nov-1988 | 20(184) |
| 08-May-2009 |  Eddie Nolan | Preston North End | Republic of Ireland | 05-Aug-1988 | 20(276) |
| 11-May-2009 | David Cotterill | Sheffield United | Wales | 04-Dec-1987 | 21(159) |
| 09-May-2009 | Chris McCann | Burnley | Republic of Ireland | 21-Jul-1987 | 21(293) |
| 08-May-2009 | Arturo Lupoli | Sheffield United | Italy | 24-Jun-1987 | 21(319) |
| 09-May-2009 | Martin Paterson | Burnley | Northern Ireland | 10-May-1987 | 21(365) |
| 08-May-2009 | Billy Jones | Preston North End | England | 24-Mar-1987 | 22(46) |
| 09-May-2009 | Shane Long | Reading | Republic of Ireland | 22-Jan-1987 | 22(108) |
| 08-May-2009 | Jamie Ward | Sheffield United | Northern Ireland | 12-May-1986 | 22(362) |
| 08-May-2009 | Stephen Quinn | Sheffield United | Republic of Ireland | 01-Apr-1986 | 23(37) |
| 09-May-2009 | Chris Eagles | Burnley | England | 19-Nov-1985 | 23(171) |
| 08-May-2009 | Ross Wallace | Preston North End | Scotland | 23-May-1985 | 23(350) |
| 09-May-2009 | Andre Amougou | Reading | Cameroon | 08-Jan-1985 | 24(121) |
| 08-May-2009 | Sean St Ledger-Hall | Preston North End | Republic of Ireland | 28-Dec-1984 | 24(131) |
| 08-May-2009 | Chris Brown | Preston North End | England | 11-Dec-1984 | 24(148) |
| 08-May-2009 | Greg Halford | Sheffield United | England | 08-Dec-1984 | 24(151) |
| 09-May-2009 | Liam Rosenior | Reading | England | 09-Jul-1984 | 24(304) |
| 09-May-2009 | Jay Tabb | Reading | Republic of Ireland | 21-Feb-1984 | 25(78) |
| 09-May-2009 | Jimmy Kebe | Reading | Mali | 19-Jan-1984 | 25(111) |
| 11-May-2009 | Craig Beattie | Sheffield United | Scotland | 16-Jan-1984 | 25(116) |
| 08-May-2009 | Matthew Kilgallon | Sheffield United | England | 08-Jan-1984 | 25(121) |
| 09-May-2009 | Dan Harding | Reading | England | 23-Dec-1983 | 25(138) |
| 08-May-2009 | Darren Carter | Preston North End | England | 18-Dec-1983 | 25(142) |
| 08-May-2009 | Andy Lonergan | Preston North End | England | 19-Oct-1983 | 25(202) |
| 09-May-2009 | Kevin Doyle | Reading | Republic of Ireland | 18-Sep-1983 | 25(234) |
| 08-May-2009 | Brian Howard | Sheffield United | England | 23-Jan-1983 | 26(106) |
| 08-May-2009 | Neil Mellor | Preston North End | England | 04-Nov-1982 | 26(186) |
| 08-May-2009 | Jon Parkin | Preston North End | England | 30-Dec-1981 | 27(129) |
| 09-May-2009 | Marek Matejovsky | Reading | Czech Republic | 20-Dec-1981 | 27(140) |
| 08-May-2009 | Nick Montgomery | Sheffield United | England | 28-Oct-1981 | 27(192) |
| 08-May-2009 | Darius Henderson | Sheffield United | England | 07-Sep-1981 | 27(243) |
| 09-May-2009 | Kalifa Cisse | Reading | Mali | 01-Sep-1981 | 27(250) |
| 09-May-2009 | Steven Caldwell | Burnley | Scotland | 12-Sep-1980 | 28(239) |
| 11-May-2009 | Leigh Bromby | Sheffield United | England | 02-Jun-1980 | 28(343) |
| 09-May-2009 | Johannes Gudjonsson | Burnley | Iceland | 25-May-1980 | 28(349) |
| 08-May-2009 | Chris Sedgwick | Preston North End | England | 28-Apr-1980 | 29(10) |
| 09-May-2009 | Dave Kitson | Reading | England | 21-Jan-1980 | 29(109) |
| 09-May-2009 | Clarke Carlisle | Burnley | England | 14-Oct-1979 | 29(208) |
| 08-May-2009 | Youl Mawene | Preston North End | France | 16-Jul-1979 | 29(297) |
| 12-May-2009 | Wade Elliott | Burnley | England | 14-Dec-1978 | 30(150) |
| 09-May-2009 | Steven Thompson | Burnley | Scotland | 14-Oct-1978 | 30(208) |
| 08-May-2009 | Barry Nicholson | Preston North End | Scotland | 24-Aug-1978 | 30(258) |
| 08-May-2009 | Paddy Kenny | Sheffield United | Republic of Ireland | 17-May-1978 | 30(357) |
| 09-May-2009 | Michael Duff | Burnley | Northern Ireland | 11-Jan-1978 | 31(118) |
| 08-May-2009 | Chris Morgan | Sheffield United | England | 09-Nov-1977 | 31(181) |
| 08-May-2009 | Paul McKenna | Preston North End | England | 20-Oct-1977 | 31(200) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).