| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 12-Aug-2008 |  Marek Suchy | Slavia Prague | Czech Republic | 28-Mar-1988 | 20(137) |
| 12-Aug-2008 |  Zdravko Kuzmanovic | Fiorentina | Serbia | 22-Sep-1987 | 20(325) |
| 12-Aug-2008 | Petr Janda | Slavia Prague | Czech Republic | 05-Jan-1987 | 21(220) |
| 12-Aug-2008 | Dani Osvaldo | Fiorentina | Italy | 12-Jan-1986 | 22(213) |
| 12-Aug-2008 | Dusan Svento | Slavia Prague | Slovakia | 01-Aug-1985 | 23(11) |
| 12-Aug-2008 | Martin Latka | Slavia Prague | Czech Republic | 28-Sep-1984 | 23(318) |
| 12-Aug-2008 | Giampaolo Pazzini | Fiorentina | Italy | 02-Aug-1984 | 24(10) |
| 12-Aug-2008 | Marek Jarolim | Slavia Prague | Czech Republic | 21-May-1984 | 24(83) |
| 12-Aug-2008 | Mickael Tavares | Slavia Prague | Senegal | 25-Oct-1982 | 25(292) |
| 12-Aug-2008 | Alberto Gilardino | Fiorentina | Italy | 05-Jul-1982 | 26(39) |
| 12-Aug-2008 | Mario Santana | Fiorentina | Argentina | 23-Dec-1981 | 26(233) |
| 12-Aug-2008 | Zdenek Senkerik | Slavia Prague | Czech Republic | 19-Dec-1980 | 27(236) |
| 12-Aug-2008 | Massimo Gobbi | Fiorentina | Italy | 31-Oct-1980 | 27(285) |
| 12-Aug-2008 | Sebastian Frey | Fiorentina | France | 18-Mar-1980 | 28(147) |
| 12-Aug-2008 | Per Kroldrup | Fiorentina | Denmark | 31-Jul-1979 | 29(13) |
| 12-Aug-2008 | Dario Dainelli | Fiorentina | Italy | 09-Jun-1979 | 29(65) |
| 12-Aug-2008 | Adrian Mutu | Fiorentina | Romania | 08-Jan-1979 | 29(217) |
| 12-Aug-2008 | Matej Krajcik | Slavia Prague | Slovakia | 19-Mar-1978 | 30(147) |
| 12-Aug-2008 | Erich Brabec | Slavia Prague | Czech Republic | 24-Feb-1977 | 31(170) |
| 12-Aug-2008 | David Hubacek | Slavia Prague | Czech Republic | 23-Feb-1977 | 31(170) |
| 12-Aug-2008 | Martin Vaniak | Slavia Prague | Czech Republic | 04-Oct-1970 | 37(313) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).