| Players | Total | Start | Sub | CO | Off | Goals |
|---|---|---|---|---|---|---|
 Marco Borriello | 35 | 25 | 13 | 10 | 11 | 11 |
John Arne Riise | 33 | 33 | - | - | - | - |
Marco Cassetti | 33 | 32 | 1 | 1 | 7 | - |
Francesco Totti | 33 | 30 | 5 | 3 | 10 | 16 |
Jeremy Menez | 33 | 24 | 12 | 9 | 18 | 2 |
Juan | 30 | 28 | 4 | 2 | 2 | 2 |
Daniele De Rossi | 29 | 29 | 1 | - | 4 | 2 |
Mirko Vucinic | 29 | 23 | 9 | 6 | 12 | 10 |
Nicolas Burdisso | 28 | 28 | 4 | - | - | 2 |
Matteo Brighi | 26 | 13 | 22 | 13 | 3 | 1 |
Simone Perrotta | 25 | 22 | 4 | 3 | 9 | 3 |
Fabio Simplicio | 25 | 19 | 16 | 6 | 7 | 4 |
Philippe Mexes | 23 | 22 | 2 | 1 | 2 | 1 |
Rodrigo Taddei | 23 | 12 | 13 | 11 | 5 | - |
David Pizarro | 22 | 21 | 7 | 1 | 3 | 1 |
Julio Sergio | 19 | 19 | 6 | - | - | - |
Aleandro Rosi | 17 | 7 | 20 | 10 | 4 | 1 |
Doni | 16 | 14 | 11 | 2 | - | - |
Leandro Greco | 14 | 9 | 15 | 5 | 6 | - |
Julio Baptista | 7 | - | 12 | 7 | - | - |
Bogdan Lobont | 6 | 6 | 22 | - | - | - |
Cicinho | 6 | 3 | 11 | 3 | 3 | - |
Simone Loria | 6 | 2 | 9 | 4 | - | 2 |
Adriano | 5 | 2 | 8 | 3 | 2 | - |
Paolo Castellazzi | 4 | 3 | 14 | 1 | - | - |
Stefano Okaka | 4 | - | 6 | 4 | - | - |
Paolo Castellini | 3 | 2 | 8 | 1 | 1 | - |
Guillermo Burdisso | 3 | 1 | 10 | 2 | - | - |
Gianluca Caprari | 1 | - | 5 | 1 | - | - |
Stefano Pettinari | - | - | 1 | - | - | - |
Federico Viviani | - | - | 1 | - | - | - | 538 | 429 | 272 | 109 | 109 | 58 |
Total = Total appearances,
Start = Total started,
CO = Came on,
Sub = Started as a sub,
Off = Taken off .