| MD | Players | Clubs | Nationality | DOB | A(D) |
|---|---|---|---|---|---|
| 19-Sep-2020 |  Finley Hurford-Lockett | Bolton Wanderers | England | 10-Apr-2003 | 17(163) |
| 12-Sep-2020 |  Marley Marshall | Colchester United | England | 22-Oct-2002 | 17(326) |
| 12-Sep-2020 |  Ethan Walker | Carlisle United | England | 28-Jul-2002 | 18(47) |
| 19-Sep-2020 |  Joseph Starbuck | Grimsby Town | England | 03-Aug-2002 | 18(48) |
| 12-Sep-2020 | Duncan Idehen | Grimsby Town | England | 03-Jul-2002 | 18(72) |
| 19-Sep-2020 |  Finn Cousin-Dawson | Bradford City | England | 04-Jul-2002 | 18(78) |
| 12-Sep-2020 | Reece Staunton | Bradford City | England | 10-Dec-2001 | 18(277) |
| 12-Sep-2020 |  Jack Smith | Stevenage | England | 15-Sep-2001 | 18(363) |
| 12-Sep-2020 |  Joshua Griffiths | Cheltenham Town | England | 05-Sep-2001 | 19(7) |
| 12-Sep-2020 | Kwame Poku | Colchester United | England | 11-Aug-2001 | 19(32) |
| 19-Sep-2020 | Jake Young | Forest Green Rovers | England | 22-Jul-2001 | 19(59) |
| 12-Sep-2020 | Terry Taylor | Grimsby Town | Wales | 29-Jun-2001 | 19(75) |
| 12-Sep-2020 |  Terrell Egbri | Southend United | England | 21-Jun-2001 | 19(83) |
| 12-Sep-2020 |  Alfie Bates | Walsall | England | 03-May-2001 | 19(132) |
| 12-Sep-2020 | Noah Chilvers | Colchester United | England | 22-Feb-2001 | 19(202) |
| 12-Sep-2020 | Tyrese Sinclair | Mansfield Town | England | 04-Feb-2001 | 19(220) |
| 12-Sep-2020 | Jack Armer | Carlisle United | Scotland | 16-Jan-2001 | 19(239) |
| 12-Sep-2020 | Richard Taylor | Southend United | England | 02-Oct-2000 | 19(345) |
| 12-Sep-2020 | Finn Azaz | Cheltenham Town | England | 07-Sep-2000 | 20(5) |
| 12-Sep-2020 |  Ruel Sotiriou | Leyton Orient | Cyprus | 24-Aug-2000 | 20(19) |
| 12-Sep-2020 |  Will Dean | Exeter City | England | 07-Aug-2000 | 20(36) |
| 12-Sep-2020 | Danny Preston | Grimsby Town | England | 06-Aug-2000 | 20(37) |
| 12-Sep-2020 |  Lee O'Connor | Tranmere Rovers | Republic of Ireland | 28-Jul-2000 | 20(46) |
| 12-Sep-2020 | Billy Crellin | Bolton Wanderers | England | 30-Jun-2000 | 20(74) |
| 12-Sep-2020 | Udoka Godwin-Malife | Forest Green Rovers | England | 09-May-2000 | 20(126) |
| 12-Sep-2020 | Joshua Kayode | Carlisle United | Nigeria | 04-May-2000 | 20(131) |
| 19-Sep-2020 | Sammie McLeaod | Colchester United | England | 23-Apr-2000 | 20(149) |
| 12-Sep-2020 | Isaac Hutchinson | Southend United | England | 10-Apr-2000 | 20(155) |
| 19-Sep-2020 | Elliot Bonds | Cheltenham Town | Guyana | 23-Mar-2000 | 20(180) |
| 12-Sep-2020 | Elliot Watt | Bradford City | Scotland | 11-Mar-2000 | 20(185) |
| 12-Sep-2020 | Kane Wilson | Forest Green Rovers | England | 11-Mar-2000 | 20(185) |
| 12-Sep-2020 | Eren Kinali | Southend United | England | 24-Feb-2000 | 20(201) |
| 19-Sep-2020 | Ryan Cooney | Morecambe | England | 26-Feb-2000 | 20(206) |
| 19-Sep-2020 | George Lloyd | Cheltenham Town | England | 11-Feb-2000 | 20(221) |
| 12-Sep-2020 |  George Hornshaw | Scunthorpe United | England | 20-Jan-2000 | 20(236) |
| 12-Sep-2020 | Saikou Janneh | Newport County | The Gambia | 11-Jan-2000 | 20(245) |
| 12-Sep-2020 | Scott Wilson | Barrow | England | 10-Jan-2000 | 20(246) |
| 19-Sep-2020 | Brandon Cooper | Newport County | Wales | 14-Jan-2000 | 20(249) |
| 12-Sep-2020 | Jordan Bedeau | Scunthorpe United | England | 24-Dec-1999 | 20(263) |
| 12-Sep-2020 | Joshua Key | Exeter City | England | 19-Nov-1999 | 20(298) |
| 12-Sep-2020 | George Tanner | Carlisle United | England | 16-Nov-1999 | 20(301) |
| 12-Sep-2020 |  Thomas Haymer | Oldham Athletic | England | 16-Nov-1999 | 20(301) |
| 19-Sep-2020 | Leon Davies | Cambridge United | England | 22-Nov-1999 | 20(302) |
| 12-Sep-2020 | Arthur Read | Stevenage | England | 03-Nov-1999 | 20(314) |
| 12-Sep-2020 | Joel Randall | Exeter City | England | 29-Oct-1999 | 20(319) |
| 19-Sep-2020 | Lewis Freestone | Cheltenham Town | England | 26-Oct-1999 | 20(329) |
| 12-Sep-2020 | Liam Kitching | Forest Green Rovers | England | 01-Oct-1999 | 20(347) |
| 12-Sep-2020 | Jamie Cumming | Stevenage | England | 04-Sep-1999 | 21(9) |
| 12-Sep-2020 | Archie Collins | Exeter City | England | 31-Aug-1999 | 21(13) |
| 19-Sep-2020 | Tom Clifford | Southend United | England | 02-Sep-1999 | 21(18) |
MD = Match date,
DOB = Date Of Birth,
A(D) = Age(Days).